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tsg期刊前沿信息_tsgd0001-2019(2024年12月实时热点)

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tsg期刊

「转发精诚口腔医学期刊」《牙髓再生中基于外泌体的细胞归巢和血管生成分化》第一作者 Venkateswaran Ganesh Dongrim Seol 作者单位 Department of Orthopedics and Rehabilitation, University of Iowa, Iowa City, IA 52242, USA Department of Roy J. Carver Biomedical Engineering, University of Iowa, Iowa City, IA 52242, USA 通讯作者 Dongrim Seol 作者单位 Department of Orthopedics and Rehabilitation, University of Iowa, Iowa City, IA 52242, USA Department of Orthodontics, University of Iowa, Iowa City, IA 52242, USA 主要内容：这篇文章在2022年12月发表在International Journal of Molecular Sciences,SCI影响因子为5.6,JCR分区为Q1区。外泌体是细胞主动分泌的一种细胞外囊泡，在增强细胞募集、促进定向分化和组织修复上具有巨大潜力。该研究利用兔牙髓干细胞提取外泌体，探究其在牙髓再生中对细胞归巢和血管生成分化的影响。首先提取兔牙髓干细胞，通过血管内皮形成实验和成骨培养基培茜素红染色证明分离的细胞为干细胞。然后提取外泌体，通过扫描电子显微镜和抗体阵列分析TSG101、CD81等外泌体标志阳性表达证明其为外泌体。该研究通过不同浓度梯度的外泌体证明5㗱0^8/mL为最佳处理浓度，其对牙髓干细胞的细胞毒性、增殖、迁移和血管生成具有积极影响。该研究还发现含外泌体的干细胞培养基中血管生成标志物VEGFA、FLT1、PECAM1高表达，证明外泌体可有效促进牙髓干细胞血管向分化。最后该研究统计了外泌体处理后的成熟和发夹miRNA表达情况。其中的关键miRNA可诱导内皮小管的形成，促进细胞迁移和增殖分化。所以，DPSC-Exos可显著促进细胞增殖、迁移和血管向分化，同时该研究还发现了调节细胞归巢和血管生成的关键miRNA。因此，基于外泌体的细胞归巢和血管向分化策略对牙髓再生具有显著的治疗潜力。中华口腔医学会的微博视频

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